Respiration of Rat Peritoneal Mast Cells
نویسندگان
چکیده
Methods for microgasometry of a few hundred mast cells are described. The Cartesian ampulla diver technique is used. The sample size is determined by counting the cells within the diver. The respiration rates at 37 degrees C, expressed in microliters per cell per hour, are 0.29 x 10(-6) without substrate and 0.47 x 10(-6) with glucose.
منابع مشابه
Mast Cell Leukemia with Ascites and Multiple Organs Damage
Mast Cell Leukemia (MCL), a rare subtype of systemic mastocytosis is defined by bone marrow involvement as atypical and aleukemic mast cells, if more than 20% and less than 10% of peripheral WBCs are mast cells, respectively. We met a case of aleukemic MCL presenting with anemia and ascites for 2 years, referred for BM evaluation, suspicious of leukemia. Our findings included BM involveme...
متن کاملMechanisms of Immunologic Injury of Rat Peritoneal Mast Cells
The ability of a number of p-nitrophenylethyl, alkyl phenylalkyl, chloroalkyl, and aminoalkyl phosphonates to inhibit the homocytotropic antibody-mediated release of histamine from rat peritoneal mast cells has been tested. The effectiveness of these same phosphonates against the activated first component of rat complement (C'1a) has also been investigated. The rat mast cell esterase activated ...
متن کاملEffects of Acute Intraperitoneal Injection on Plural Rat Lungs
Purpose: Sulfur mustard (SM), a highly toxic chemical warfare, primarily targets the skin, eye and respiratory tract. Respiratory tract lesions are the most disabling consequences. Mast cells, containing special granules that store histamine and heparin, release leukotrienes. It is known that the surface of mast cells contains specific receptors for 19B. Therefore, mast cells promote allergic r...
متن کاملProtective effect of disodium cromoglycate on rat peritoneal mast cells.
Disruption of mast cells was measured by incubating the peritoneal washings of a rat with the substances under test. The incubate was filtered and the histamine in the undisrupted mast cells on the filter was assayed using guinea-pig ileum. Measurements were also made on disruption of rat peritoneal mast cells in vivo. Disodium cromoglycate (DCG) reduces the in vitro disruption of peritoneal ma...
متن کاملThe Lectin ArtinM Induces Recruitment of Rat Mast Cells from the Bone Marrow to the Peritoneal Cavity
BACKGROUND The D-mannose binding lectin ArtinM is known to recruit neutrophils, to degranulate mast cells and may have potential therapeutic applications. However, the effect of ArtinM on mast cell recruitment has not been investigated. METHODOLOGY Male Wistar rats were injected i.p. with ArtinM or ConA (control). The ability of the lectin to degranulate peritoneal and mesenteric mast cells w...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- The Journal of Cell Biology
دوره 25 شماره
صفحات -
تاریخ انتشار 1965